Coding
Str(e)pFG

Part:BBa_K4590010:Design

Designed by: Beatriz Lauriano de Oliveira   Group: iGEM23_USP-Brazil   (2023-10-02)


di-Tricarboxylate Transporter and Malonyl-CoA Synthase


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2374
    Illegal PstI site found at 2032
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2374
    Illegal PstI site found at 2032
    Illegal NotI site found at 1072
    Illegal NotI site found at 1213
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2374
    Illegal BamHI site found at 461
    Illegal BamHI site found at 989
    Illegal XhoI site found at 1592
    Illegal XhoI site found at 2152
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2374
    Illegal PstI site found at 2032
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2374
    Illegal PstI site found at 2032
    Illegal NgoMIV site found at 52
    Illegal NgoMIV site found at 439
    Illegal NgoMIV site found at 1692
    Illegal NgoMIV site found at 1846
    Illegal NgoMIV site found at 1924
    Illegal AgeI site found at 2137
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 291
    Illegal SapI site found at 525
    Illegal SapI site found at 774


Design Notes

We chose genes either from another species of Streptomyces or from a closely related organism like Rhizobium.


Source

Streptomyces hygroscopicus var. ascomyceticus FK520 biosynthetic gene cluster, partial sequence GenBank: AF235504.1 Rhizobium leguminosarum bv. viciae chromosome complete genome, strain 3841 GenBank: AM236080.1

References